2026 - Poster: High-throughput lysosome isolation and multi-modal functional characterization
Bastien Masson & al. Lysosomes are key organelles combining essential degradation functions, intracellular homeostasis and signalling. They are implicated in several neurodegenerative diseases, cancer and immune disorders. Despite the fact that 70% of proteins are inside the cells, so far, critical ion channels, transporters and luminal enzymes have been understudied due to technical limitations. ORIA Bioscience has developed a unique microfluidic-based isolation and conservation method to provide scientists with highly pure and functional lysosomes. ORIA’s quality control is based on combining imaging, specific protein markers presence, complemented by enzymatic activity as an additional functional readout. Further electrophysiology-based validations on distinct platforms demonstrated the improved functionality and reliability of the LYSO-prep™ for the recordings of key targets e.g. TRPML1, TMEM175 and TCP2.